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folEB [2019-06-18 12:06:38]
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folEB [2019-06-18 12:06:38]

GTP cyclohydrolase IB, replaces FolE under conditions of zinc starvation
locus
BSU03340
pI
7.83
mw
34.63 kDa
protein length
305 aa Sequence Blast
gene length
915 bp Sequence Blast
function
biosynthesis of folate
product
zinc-independent GTP cyclohydrolase IB
essential
no
ec
3.5.4.16
synonyms
folE2,yciA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.5|Biosynthesis of folate]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

    • Gene

    Coordinates
    364,259 → 365,173

    The protein

    Catalyzed reaction/ biological activity

  • GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate (according to Swiss-Prot)

    • Protein family

  • GTP cyclohydrolase IV family (according to Swiss-Prot)

    • [SW|Cofactors]

  • activated by a variety of divalent cations [Pubmed|19767425]

    • Structure

  • [PDB|3D1T] [Pubmed|19767425]

    • Expression and Regulation

    Operons

    genes
    [gene|93380E8EEB672C14C9BE2B5A6D8573FBCA149514|folEB]-[gene|456AF184261976FAA73359E81443F7035F457D07|yciB]-[gene|F735802BB483C2C5437240AB2E9FC340EAA5B353|yczL]-[gene|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA]
    description
    [Pubmed|9811636]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12426338], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]: repression, in the presence of zinc [Pubmed|12426338], in [regulon|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur regulon]

    • regulation

  • [SW|folE2]: induced by zinc starvation ([protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]) [Pubmed|12426338]
    • view in new tab

    Biological materials

    Mutant

  • BKE03340 (Δ[gene|93380E8EEB672C14C9BE2B5A6D8573FBCA149514|folEB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA, downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA
  • BKK03340 (Δ[gene|93380E8EEB672C14C9BE2B5A6D8573FBCA149514|folEB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA, downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA

    • References

  • 9811636,12426338,17032654,19767425,27561249